USE OF ROTOFOR PREPARATIVE ISOELECTROFOCUSING CELL IN PROTEIN-PURIFICATION PROCEDURE

Authors
AYALA A PARRADO J MACHADO A
Citation
A. Ayala et al., USE OF ROTOFOR PREPARATIVE ISOELECTROFOCUSING CELL IN PROTEIN-PURIFICATION PROCEDURE, Applied biochemistry and biotechnology, 69(1), 1998, pp. 11-16
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
69
Issue
1
Year of publication
1998
Pages
11 - 16
Database
ISI
SICI code
0273-2289(1998)69:1<11:UORPIC>2.0.ZU;2-J
Abstract
The Rotofor cell is a preparative isoelectric focusing (IEF) apparatus , in which IEF is performed entirely in free solution. Electrofocusing in Rotofor cell has been described as well-suited for use at any stag e of a purification scheme. However, it has some important limitations in resolving complex mixtures of proteins. This paper describes the a dvantages and disadvantages of using the Rotofor cell in purification protocols.