HUMAN DOSE-EXCRETION STUDIES WITH THE PYRETHROID INSECTICIDE CYFLUTHRIN - URINARY METABOLITE PROFILE FOLLOWING INHALATION

1. Nine male volunteers were exposed to the pyrethroid insecticide cyf luthrin. The study was performed in an exposure room, where an aerosol containing cyfluthrin was sprayed to obtain atmospheres with mean cyf luthrin concentrations of 160 and 40 mu g/m(3) Four volunteers were ex posed for 10, 30 and 60 min at 160 mu g/m(3) and another five voluntee rs were exposed for 60 min at 40 mu g/m(3) For 160 mu g/m(3) exposure urine samples were collected before and immediately after exposure as well as for the periods 1-2, 2-3, 3-4, 4-5, 5-6, 6-12 and 12-24 h afte r exposure. For 40 mu g/m(3) exposure urine samples were collected bef ore and 2 h after exposure. 2. The main urinary cyfluthrin metabolites , ans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid ( DCCA) end 4-fluoro-3-phenoxybenzoic acid (FPBA), were determined. The limit of detection (LOD) for all metabolites was 0.0025 mu g in an uri ne sample of 5 ml (0.5 mu g/l). After inhalative exposure of 40 mu g c yfluthrin/m(3) air for 60 min, the amount of metabolites in urine coll ected in the first 2 h after exposure was less than the LOD, namely 0. 14 mu g for cis-DCCA, 0.15-0.28 mu g for trans-DCCA and 0.12-0.23 mu g for FPBA. 3. Of the metabolites, 93% was excreted within the first 24 h (peak excretion rates between 0.5 and 3 h) after inhalative exposur e of 160 mu g/m(3). The mean half-lives were 6.9 h for cis-DCCA, 6.2 h for trans-DCCA and 5.3 h for FPBA.4. The mean trans-:cis-DCCA ratio w as 1.9 for the time course as well as for each subject. 5. The amount of metabolites in urine depends on the applied dose, on the exposure t ime and shows interindividual differences.