THE ROLE OF PROTEINS IN THE NUCLEATION AND FORMATION OF CALCIUM-CONTAINING DEPOSITS ON BIOMATERIAL SURFACES

Citation
Sl. Vasin et al., THE ROLE OF PROTEINS IN THE NUCLEATION AND FORMATION OF CALCIUM-CONTAINING DEPOSITS ON BIOMATERIAL SURFACES, Journal of biomedical materials research, 39(3), 1998, pp. 491-497
Citations number
30
Categorie Soggetti
Materials Science, Biomaterials
ISSN journal
00219304
Volume
39
Issue
3
Year of publication
1998
Pages
491 - 497
Database
ISI
SICI code
0021-9304(1998)39:3<491:TROPIT>2.0.ZU;2-5
Abstract
In experiments in vivo using diffusion chambers, the morphology and co mposition of calcium-containing deposits on natural and artificial bio materials that had no direct contact with cells were studied using sca nning electron microscopy with energy dispersion X-ray microanalysis. It was revealed that the formation of a protein layer containing prote in-calcium complexes is the key event in biomaterial calcification. A mechanism of formation of a calcium-containing protein matrix that cre ates the conditions for supersaturation of the crystal-forming medium over critical value has been proposed. The formation of nuclei of inso luble calcium phosphate starts predominantly deep in an adsorbed prote in layer enriched by calcium ions. (C) 1998 John Wiley & Sons, Inc.