ROLE OF THE C-9 METHYL-GROUP IN RHODOPSIN ACTIVATION - CHARACTERIZATION OF MUTANT OPSINS WITH THE ARTIFICIAL CHROMOPHORE 11-CIS-9-DEMETHYLRETINAL

Citation
M. Han et al., ROLE OF THE C-9 METHYL-GROUP IN RHODOPSIN ACTIVATION - CHARACTERIZATION OF MUTANT OPSINS WITH THE ARTIFICIAL CHROMOPHORE 11-CIS-9-DEMETHYLRETINAL, Biochemistry, 37(2), 1998, pp. 538-545
Citations number
36
Categorie Soggetti
Biology
Journal title
ISSN journal
00062960
Volume
37
Issue
2
Year of publication
1998
Pages
538 - 545
Database
ISI
SICI code
0006-2960(1998)37:2<538:ROTCMI>2.0.ZU;2-6
Abstract
Activation of the visual pigment rhodopsin involves both steric and el ectrostatic interactions between the chromophore and opsin within the retinal-binding site. Removal of the C-9 methyl group of 11-cis-retina l inhibits light-dependent activation of the G protein, transducin, su ggesting a direct steric contact. More recently, we have shown that st eric interactions lead to receptor activation when Gly(121) in the mid dle of transmembrane helix 3 is replaced by larger hydrophobic residue s. In order to understand in more detail the role of the C-9 methyl gr oup of retinal in the structure and function of rhodopsin, we first st udied the properties of recombinant 9-dm-Rho (opsin reconstituted with 11-cis-9-demethylretinal). The 9-dm-Rho pigment displayed a blue-shif ted lambda(max), increased hydroxylamine reactivity, and decreased abi lity to activate transducin. These properties are consistent with the hypothesis that the C-9 methyl group is a crucial structural anchor or the correct docking of the chromophore in its binding site. Next, we investigated the possible interaction between Gly(121) of opsin and th e C-9 methyl group of retinal by characterizing recombinant pigments p roduced by combining mutant opsins (G121A, -V, -I, -L, and -W) with 11 -cis-9-demethylretinal. Mutant opsins G121I, -L, and -W failed to bind the chromophore. However, the double mutant G121L/F261A bound 11-cis- 9-demethylretinal to form a stable pigment with a lambda(max) of 451 n m. When activity was assayed in membranes, the reduction in transducin activation by 9-dm-Rho caused by the lack of a C-9 methyl group on th e chromophore could be partially restored by replacing Gly(121) with a bulky residue (leucine, isoleucine, or tryptophan). These results sup port a model of receptor activation that involves steric interaction b etween the C-9 methyl group of the chromophore and the opsin in the vi cinity of Gly(121) on transmembrane helix 3.