EFFECT OF COLONIC OBSTRUCTION ON THE DISTRIBUTION OF MATRIX METALLOPROTEINASES DURING ANASTOMOTIC HEALING

Citation
Fj. Savage et al., EFFECT OF COLONIC OBSTRUCTION ON THE DISTRIBUTION OF MATRIX METALLOPROTEINASES DURING ANASTOMOTIC HEALING, British Journal of Surgery, 85(1), 1998, pp. 72-75
Citations number
18
Categorie Soggetti
Surgery
Journal title
ISSN journal
00071323
Volume
85
Issue
1
Year of publication
1998
Pages
72 - 75
Database
ISI
SICI code
0007-1323(1998)85:1<72:EOCOOT>2.0.ZU;2-S
Abstract
Background Anastomotic dehiscence is common after surgery for colonic obstruction. The strength of an anastomosis is dependent on collagen, which is degraded by matrix metalloproteinases (MMPs). The aim of this study was to determine the distribution of the MMPs and their inhibit or, tissue inhibitor of metalloproteinases (TIMP) 1 in an experimental model of colonic obstruction, with and without resection and anastomo sis. Methods The distal colon of rabbits was obstructed with a Silasti c ring for 24 h and then either the ring was removed or the obstructed segment was resected and an anastomosis formed. Rabbits were killed i mmediately or at intervals for up to 7 days after operation. The distr ibution of the MMPs and TIMP-1 was examined by indirect immunofluoresc ence. Results MMPs and TIMP-1 were present throughout the descending c olon for 24 h in both groups. They persisted to the third day in rabbi ts with an anastomosis but by day 7 were restricted to the suture line . Their presence correlated with microscopic damage. Conclusion The ex tensive distribution of the MMPs suggests that these enzymes contribut e to anastomotic dehiscence, but only in the immediate postoperative p eriod.