K. Taulavuori et al., MODELING FROST-RESISTANCE OF SCOTS PINE-SEEDLINGS USING TEMPERATURE, DAYLENGTH AND PH OF CELL EFFUSATE, Plant ecology, 133(2), 1997, pp. 181-189
The annual course of frost resistance (LT50) and the pH of the cell ef
fusate in needles of two-year-old Scots pine seedlings were monitored
in a field experiment in Oulu, Northern Finland (65 degrees N, 25 degr
ees E) during 1995. The aim of the work was to to develop model to pre
dict the annual variation in frost resistance by pH of the cell effusa
te and meteorological data. The seedlings were covered with a fibre cl
oth shelter which transmitted sufficient Light for them to experience
the photoperiod, but prevented the accumulation of snow over them. The
shelter above the seedlings was removed at the beginning of May and e
rected again at the end of September. the seedlings were watered only
for the time when the shelter was removed, and received fertilizer onl
y during the previous summer (1994). Frost resistance was only -5 degr
ees C during the growing season but more than -100 degrees C during th
e winter rest period. It was about -10 degrees C at the end of August,
increased to -55 degrees C in the next three weeks, and reached -100
degrees C at the beginning of October. The pH of the cell effusate was
lowest during the growing season and highest in winter, the differenc
e being about one and half pH unit. Needles exposed to -196 degrees C
showed pH from 4.0 in summer to 5.5 in winter, while pH of the non-fro
zen needles varied from 5.0 to 6.5, respectively. Seasonal variation i
n frost resistance was explained by a regression model well (R-2 = 0.9
) when day length, minimum air temperature and pH were entered as vari
ables.