LECTIN-INDUCED INCREASE IN CLONOGENIC GROWTH OF HEMATOPOIETIC PROGENITOR CELLS

The galactoside-specific plant lectin, Viscum album agglutinin (VAA-I) increases cellular parameters of natural host defence. It also binds to a variety of haematopoietic cells, including progenitors. We invest igated whether VAA-I has a stimulatory effect on haematopoietic progen itor cells. Peripheral blood progenitor cells from 7 healthy volunteer s were cultured in a colony assay with VAA-I plus erythropoietin (EPO) and stem cell factor (SCF). At 50 pg/ml VAA-I induced a significant i ncrease in the cytokine-dependent clonogenic growth (52% in median, p < 0.05). In another set of experiments purified CD34(+) cells were iso lated from the bone marrow aspirate of 4 patients with non-metastatic breast cancer using fluorescence-activated cell sorting. Binding to CD 34(+) cells was demonstrated by using directly fluorescence-conjugated VAA-I. Go-incubation with D-galactose significantly abrogated this ef fect, CD34(+) cells were cultured in the presence of EPO, SCE interleu kin-3, granulocyte/monocyte colony-stimulating factor and granulocyte colony-stimulating factor. VAA-I alone had no measurable effect on the clonogenic growth of the isolated cells. However, at concentrations o f 100 and 250 pg/ml VAA-I increased the cytokine-dependent proliferati on and differentiation of CD34(+) cells by a median of 75 and 85%, res pectively. The results show that VAA-I binds to haematopoietic progeni tor cells and has a co-stimulatory effect on their proliferation.