AMINO-ACID AND PROTEIN-CHANGES IN TILAPIA AND SPANISH MACKEREL AFTER IRRADIATION AND STORAGE

Some amino acids in tilapia decreased while some others increased when subjected to doses up to 10.0 kGy. However, 10 kGy contributed to a s ignificant reduction in all amino acids of Spanish mackerel. Variation s in amino acid contents continued during post-irradiation storage wit h no consistant trend of increase or decrease. SDS-PAGE of protein fro m both fish showed 27 bands of subunits with MW < 14.0-94.0 KD. Isoele ctric focusing patterns of sarcoplasmic protein of unirradiated and ir radiated fish showed no change in the number of bands, while some chan ges were observed in the intensities of the anodic and cathodic bands depending on isoelectric points (pIs). (C) 1998 Elsevier Science Ltd. All rights reserved.