A SERIES OF VECTORS TO CONSTRUCT LACZ FUSIONS FOR THE STUDY OF GENE-EXPRESSION IN SCHIZOSACCHAROMYCES-POMBE

We have constructed a series of plasmids to facilitate the fusion of p romoters with or without coding regions of genes of Schizosaccharomyce s pombe to the lacZ gene of Escherichia coli. These vectors carry a mu ltiple cloning region in which fission yeast DNA mag be inserted in th ree different reading frames,vith respect to the coding region of lacZ . The plasmids mere constructed with the ura4(+) or the his3(+) marker of S. pombe. Functionality of the plasmids was tested measuring in pa rallel the expression of fructose 1,6-bisphosphatase and beta-galactos idase under the control of the fbp1(+) promoter in different condition s. (C) 1997 Federation of European Biochemical Societies.