K. Yanagisawa et al., AMYLOID BETA-PROTEIN (A-BETA) ASSOCIATED WITH LIPID MOLECULES - IMMUNOREACTIVITY DISTINCT FROM THAT OF SOLUBLE A-BETA, FEBS letters, 420(1), 1997, pp. 43-46
We previously identified a novel amyloid P-protein (A beta), that bind
s to GM1 ganglioside, in brains exhibiting the early pathological chan
ges of AD, In this study, we raised monoclonal antibodies, using membr
ane fractions containing abundant GM1 ganglioside-bound A beta as anti
gens, Monoclonal antibody 4396, produced in this study, immunoprecipit
ates A beta 42 in the membrane fractions of brains with diffuse plaque
s, but does not react with soluble A beta 42 or GM1 ganglioside, Furth
ermore, this antibody recognizes the AP bound to lipid vesicles contai
ning GM1 ganglioside, and unexpectedly, phosphatidylinositol. In contr
ast, a control anti-A beta monoclonal antibody does not recognize the
A beta bound to these lipid vesicles, These results indicate that A be
ta associated with lipids has an immunoreactivity distinct from that o
f soluble A. (C) 1997 Federation of European Biochemical Societies.