FUNCTIONAL EXPRESSION OF PHARAONIS PHOBORHODOPSIN IN ESCHERICHIA-COLI

Pharaonis phoborhodopsin, the photoreceptor of the negative phototaxis of archaebacterial. Natronobacterium pharaonis, was functionally expr essed in the heterologous system of Escherichia coli. Flash-photolysis on a millisecond time scale indicated that the photochemical properti es of ppR expressed in E. coli were the same as those of the native pp R in N. pharaonis. Wt concluded that the integral membrane protein ppR is correctly folded in vivo in the eubacterial E, coli membrane. (C) 1997 Federation of European Biochemical Societies.