NEW CHICKEN RFP-Y HAPLOTYPES ON THE BASIS OF MHC CLASS-II RFLP AND MLC ANALYSES

New chicken Rfp-Y haplotypes were determined by the use of restriction fragment length polymorphism (RFLP) and mixed lymphocyte culture (MLC ) in four different chicken haplotypes, B-15, B-19, B-21, B-201. The R FLP polymorphism was mapped to the Rfp-Y system by the use of a subclo ne (18.1) which maps near a polymorphic lectin gene located in the Rfp -Y system and DNA from families with known segregation of the implicat ed RFLP polymorphism. For the first time it is shown that major histoc ompatibility complex class II genes in the Rfp-Y system have functiona l implications. Sequence information of the B1 domain of the proposed Rfp-Y haplotypes was obtained which supported the functional data.