MULTIPARAMETER ANALYSIS OF VASCULATURE, PERFUSION AND PROLIFERATION IN HUMAN TUMOR XENOGRAFTS

A method is presented in this report for concurrent analysis of vascul ar architecture, blood perfusion and proliferation characteristics in whole-tumour cross-sections of human larynx carcinoma and glioblastoma xenografts. Tumours were implanted subcutaneously in nude mice. After i.v. injection with Hoechst 33342 and bromodeoxyuridine (BrdUrd) as p erfusion and proliferation markers, animals were killed. An antiendoth elial antibody (9F1) was used to delineate vascular structures. Cross- sections were analysed by a multistep immune staining and a computer-c ontrolled microscope scanning method. Each tumour section was stained and scanned four times (Hoechst, 9F1, BrdUrd and Fast Blue for all nuc lei). When these images were combined, vasculature, perfusion and prol iferation parameters were analysed. The labelling index (LI) was defin ed as the ratio of the BrdUrd-labelled area to the total nuclear area. The LI based on manual counting and the LI calculated by flow cytomet ry (FCM) were in good agreement with the LI based on surface analysis. LI decreased at increasing distance from its nearest vessel. In the v icinity of perfused vessels, the LI was 30-70% higher than near non-pe rfused vessels. This method shows that both vasculature/perfusion and proliferation characteristics can be measured in the same whole-tumour section in a semiautomatic way. This could be applied in clinical pra ctice to identify combined human tumour characteristics that predict f or a favourable response to treatment modifications.