EFFECTS OF GENISTEIN AND LAVENDUSTIN ON REPRODUCTIVE PROCESSES IN DOMESTIC-ANIMALS IN-VITRO

The aim of our experiments was to study the influence of genistein [ty rosine kinase (TK) inhibitor with estrogenic activity] and lavendustin A (TK inhibitor without estrogenic activity) on female reproductive p rocesses in domestic animals in vitro. It was found that genistein (0. 001-1 mu g/ml) increased IGF-I release by cultured bovine and porcine granulosa cells, but decreased its secretion by rabbit granulosa cells (0.01-10 mu g/ml). Genistein stimulated progesterone secretion by bov ine and rabbit granulosa cells (at 0.01-10 mu g/ml), estradiol output by rabbit granulosa cells (at 1 mu g/ml) and porcine ovarian follicles (at 10 mu g/ml), as web as cAMP production by bovine (at 0.001-1 mu g /ml) and rabbit (at 1 mu g/ml) granulosa cells. No effects of genistei n (at 10 mu g/ml) on PGF-2 alpha and progesterone release by porcine o varian follicles were observed. Genistein significantly (P < 0.05) sti mulated the reinitiation and completion of nuclear maturation of porci ne oocytes (at 5 mu g/ml), as well as the preimplantation development of rabbit zygotes (at 1 mu g/ml). Lavendustin A (0.001-1 mu g/ml) incr eased IGF-I release by bovine (but not by porcine) granulosa cells, cA MP release by bovine granulosa cells, and PGF-2 alpha output by porcin e ovarian follicles (at 10 mu g/ml). Lavendustin (at 1 mu g/ml) had no significant effect on IGF-I release by porcine granulosa cells, on es tradiol and cAMP output by rabbit granulosa cells, or on progesterone secretion by porcine follicles (at 10 mu g/ml). Inhibitory actions of lavendustin (at 10 mu g/ml) on estradiol secretion by porcine follicle s were also found. Furthermore, lavendustin, like genistein, promoted the reinitiation and completion of meiosis in porcine oocytes. The pre sent study demonstrates a predominantly stimulatory effect of TK inhib ition on endocrine and generative processes in domestic animals. The m ajority of these effects are similar for both compounds, indirectly su ggesting that their action is due to tyrosine kinase inhibition and pr otein kinase A-stimulation, rather than estrogenic activity. (C) 1997 Published by Elsevier Science Ltd. All rights reserved.