Objectives: To assess the in vitro and in vivo accuracy of a mathemati
cal computer-based reconstruction of two images that are not taken wit
h the same recording geometry for the measurement of apical root resor
ption following orthodontic treatment. Methods: A gold standard for ro
ot resorption in vitro was developed from 10 extracted upper central i
ncisors using calipers. Radiographs made with five different projectio
n angles were reconstructed mathematically by two observers. The calcu
lated loss of length was compared with the gold standard. Eighty-two u
pper central incisors from 61 patients were radiographically evaluated
for the prevalence and degree of apical root resorption after orthodo
ntic fixed appliance therapy. The relative amount of reduction was cal
culated after mathematical reconstruction. Results: The inter-observer
error in vitro was 1.8%. The 95% confidence intervals for the differe
nce with the gold standard are small. The duplicate measurement in viv
o error was 2.2% and the correlation between duplicate measurements wa
s 0.94. The mean loss of tooth length was 7.8% (s.d. 6.9). Conclusions
: The prevalence of root resorption corresponds well with that in the
literature. Digital reconstruction is a reliable method to correct for
different projection angles and to monitor the effects of orthodontic
movement in serial dental radiographs.