Traditional as well as biotechnological processing of a heterogeneous
adduct like coal will lead to a complex mixture of various unknown com
pounds, The implementation of a chemical-by-chemical approach as made
possible by the development of more specific chemical and physical ana
lytics is only a limited tool to protect the environment against hazar
dous discharges. Therefore, investigations carried out were focused on
selection, adaption and validation of bioassays suited for a sensitiv
e and reproducible valuation of coal relevant products. Bioassays with
organisms representing different trophic levels like bacteria, daphni
a and algae were used for determination of ecotoxicological/toxicologi
cal effects. The obtained results show the suitability of these bioass
ays for the valuation of ecotoxicological effects of coal-relevant sub
stances and different model compounds similar to microbial transformat
ion/conversion products of coal. (C) 1997 Elsevier Science B.V.