A MAJOR 170 KDA PROTEIN ASSOCIATED WITH BOVINE ADRENAL-MEDULLA MICROTUBULES - A MEMBER OF THE CENTROSOMIN FAMILY

Microtubules isolated from bovine adrenal medulla cells contain a majo r 170 kDa protein (p170), p170 is heat-labile and is associated with m icrotubules in an ATP-insensitive manner, This protein was purified to near homogeneity using FPLC. A preparation containing purified p170 c aused bundling of microtubules. By microsequencing of p170, two polype ptides were identified which appeared to be identical to a recently se quenced p167 centrosomin-related protein, Polyclonal affinity-purified anti-p170 antibody mas found to immunostain microtubules and to recog nize the 170 kDa polypeptide in culture cells, We suggest that p170 is a nem member of a centrosomin family and is a new structural protein associated with microtubules in some cell types. (C) 1997 Federation o f European Biochemical Societies.