P53 EXPRESSION AND P53 GENE MUTATION IN ORAL-CANCER AND DYSPLASIA

BACKGROUND: The aim of the current study was to examine the possible a ssociation of the p53 tumor suppressor gene with the development of or al cancer, We examined biopsy material from patients with oral squamou s cell carcinoma for p53 protein expression and p53 mutations, METHODS : Eighteen samples were analyzed. These comprised oral squamous cell c arcinoma biopsy samples and oral dysplastic biopsy material taken from nine patients. An immunohistochemical technique was used to determine p53 protein expression. A direct sequencing technique was used to det ect mutations in the p53 gene itself, RESULTS: Overall seven of nine ( 77%) oral squamous cell carcinomas and seven of nine (77%) oral dyspla stic lesions analyzed showed evidence of p53 protein over-expression. Mutations in exons 5 and 6 of the p53 gene were detected in five of ni ne (55%) oral squamous cell carcinomas and four of nine (44%) dysplast ic lesions analyzed. CONCLUSIONS: Both p53 protein over-expression and p53 mutations were noted in dysplastic oral lesions. Over-expression of the p53 protein and p53 gene mutations were not always coexistent, however, suggesting that gene mutation may be only one of the mechanis ms responsible for stabilization of the p53 protein.