Ms. Tian et al., ETHYLENE BIOSYNTHESIS AND RESPIRATION IN STRAWBERRY FRUIT TREATED WITH DIAZOCYCLOPENTADIENE AND IAA, Plant growth regulation, 23(3), 1997, pp. 195-200
Diazocyclopentadiene (DACP), an inhibitor of ethylene action, was used
to investigate the role of ethylene receptor(s) in regulating ethylen
e biosynthesis and respiration in strawberry fruit. DACP stimulated et
hylene production of fruit at all stages of maturity. This stimulation
was mainly due to an increase in ACC content in the tissue without si
gnificantly changing ACC oxidase activity. DACP did not induce any cha
nge in respiration in fruit at various stages of maturity except the e
arly green stage (green I). We also investigated the possible interact
ion of DACP and IAA in ethylene production. Results indicated that all
treatments increased ethylene production compared to the control alth
ough the absolute ethylene production differed in the order IAA plus D
ACP > only DACP > only IAA > control. IAA stimulated ethylene producti
on without change of ACC oxidase activity at 1 day after treatment in
strawberry fruit at pink stage. Results suggest that ethylene biosynth
esis in nonclimacteric strawberry fruit at various stages of maturity
may be regulated by ethylene receptor(s) with inhibition of ethylene p
roduction. DACP may release this inhibitory effect, and resulting in i
ncreasing ethylene production. Results also indicated that respiration
may not be regulated by an ethylene receptor in strawberry fruit at m
ost stages of maturity. DACP and IAA showed interaction in regulation
of ethylene production which was caused by an increase in ACC content,
not ACC oxidase activity.