A. Callebaut et al., STABILITY OF ANTHOCYANIN COMPOSITION IN AJUGA-REPTANS CALLUS AND CELL-SUSPENSION CULTURES, Plant cell, tissue and organ culture, 50(3), 1997, pp. 195-201
Callus cultures of Ajuga reptans flowers produced a complex mixture of
cyanidin- and delphinidin-based pigments, of which more than 90% were
acylated. The anthocyanin composition varied little during one growth
period. During a time span of 5 years no new anthocyanin classes appe
ared. Quantitative differences in anthocyanin composition between the
callus lines and during a 5 year time span were more pronounced. In ge
neral, the accumulation of delphinidin-based anthocyanins decreased. T
he percentage of acylated anthocyanins was stable in time. The accumul
ation of metabolically evolved anthocyanins (5'-substituted and acylat
ed) decreased during passage from solid culture to liquid culture. The
accumulation of acylated anthocyanins was influenced by the type of a
eration in liquid cultures.