LOCALIZATION OF 14-3-3-PROTEIN IN THE NUCLEI OF ARABIDOPSIS AND MAIZE

It has been demonstrated that 14-3-3 proteins are present in the nucle i of Arabidopsis thaliana and Zea mays cells using laser scanning conf ocal microscopy and immunocytochemistry with monoclonal antibodies aga inst plant 14-3-3 proteins. Confirmation of nuclear localization provi des insight into the range of functions normally attributed to 14-3-3 proteins, especially since the association of 14-3-3s with transcripti on factors is (thus far) a phenomenon unique to plants, and since 14-3 -3 proteins do not possess a recognizable nuclear targeting sequence.