MOBILE GENETIC ELEMENT OF BORDETELLA-PERT USSIS CHROMOSOME STIMULATESCOINTEGRATE FORMATION IN ESCHERICHIA-COLI STRAINS

This work continues a series of reports devoted to the study of a repe ated sequence isolated from the Bordetella pertussis chromosome (RSBP- Repeated Sequence of Bordetella Pertussis). The RSBP was earlier demon strated to have a structure similar to IS elements and exhibit some pr operties of mobile genetic elements. The results of this work demonstr ate the ability of this novel genetic element to stimulate the formati on of cointegrates between independent replicons. We have studied the structure of five cointegrates formed by integration of a resident RSB P-containing plasmid into three different sites of a recipient replico n. The studied cointegrates are capable of resolution with the release of a resident plasmid independent of the host cell RecA function.