BIFUNCTIONAL PLANT DEFENSE ENZYMES WITH CHITINASE AND RIBOSOME-INACTIVATING ACTIVITIES FROM TRICHOSANTHES KIRILOWII CELL-CULTURES

Authors
SHIH NR MCDONALD KA JACKMAN AP GIRBES T IGLESIAS R
Citation
Nr. Shih et al., BIFUNCTIONAL PLANT DEFENSE ENZYMES WITH CHITINASE AND RIBOSOME-INACTIVATING ACTIVITIES FROM TRICHOSANTHES KIRILOWII CELL-CULTURES, PLANT SCI, 130(2), 1997, pp. 145-150
Citations number
34
Categorie Soggetti
Plant Sciences
Journal title
PLANT SCIENCE
ISSN journal
01689452 → ACNP
Volume
130
Issue
2
Year of publication
1997
Pages
145 - 150
Database
ISI
SICI code
0168-9452(1997)130:2<145:BPDEWC>2.0.ZU;2-J
Abstract
Three bifunctional plant enzymes (named TKC 15, TKC 28-I, and TKC 28-I I) with both chitinase and RIP activity were purified from the medium of Trichosanthes kirilowii plant cell cultures. Highly purified prepar ations of these proteins exhibited endochitolytic activity as well as the specific 28S rRNA N-glycosidase activity characteristic of ribosom e inactivating proteins (RIPs). In addition to providing a competitive advantage to the plant, these enzymes offer the possibility of improv ed antifungal protection when expressed in transgenic plants. (C) 1997 Elsevier Science Ireland Ltd.