OXIDATIVE BIOMACROMOLECULAR DAMAGE FROM NOVEL PHTHALOCYANINE

Photosensitized oxidation of the novel phthalocyanine modified by glyc ine [ZnPc(NHCH2COOH)(4)] in the presence of glucose oxidase or plasmid pBR322 DNA was investigated by using gel electrophoresis, capillary e lectrophoresis and the enzyme activity determination method. As a resu lt, glucose oxidase was cleaved partially by photosensitizer, the cros s-linkage between subunits occurred partially simultaneously and, more over, enzyme was cleaved continually into pieces. Activity of glucose oxidase was 130 times lower than that of original within 12 h. DNA cou ld be cleaved in the presence of photosensitizer ZnPc(NHCH2COOH)(4). S uper helix of DNA was depolymerized into nicked and linear DNA. Then, it was cleaved progressively into pieces. It was suggested that the no vel photosensitizer could do apparent damage to biomacromolecules.