CAPILLARY ELECTROPHORETIC SEPARATION OF GLYCOPROTEINS

Authors
Citation
Y. Chen, CAPILLARY ELECTROPHORETIC SEPARATION OF GLYCOPROTEINS, Science in China. Series B, Chemistry, life sciences & earth sciences, 41(1), 1998, pp. 71-76
Citations number
9
Categorie Soggetti
Chemistry
ISSN journal
1001652X
Volume
41
Issue
1
Year of publication
1998
Pages
71 - 76
Database
ISI
SICI code
1001-652X(1998)41:1<71:CESOG>2.0.ZU;2-2
Abstract
A capillary electrophoretic approach has been developed for analyzing the microheterogeneity of glycoproleins. The key factors include the b uffer composition, concentration, pH, and the surface treatment of the capillaries used. It has been shown that, if the buffer is mainly com posed of alcohol amines and the capillaries are coated with polyacryla mide, ovalbumin from chicken egg can be separated into more than 25 pe aks and that of transferrin about 10 peaks.