MODELING COLUMN REGENERATION EFFECTS ON ION-EXCHANGE CHROMATOGRAPHY

The effect of in-place regeneration on equilibrium and kinetic charact eristics of the adsorption of bovine serum albumin to a DEAE-cellulose anion exchanger has been determined. Regeneration with sodium hydroxi de and time of exposure showed no effect on equilibrium behavior. Brea kthrough curves were measured for protein adsorption on fixed-bed colu mns and analyzed by a simple model to determine the relevant rate cons tants for the adsorption process. It was found that forward adsorption rate constant decreased exponentially with the chemical treatment exp osure time. The implications of the results on the design and optimiza tion of ion-exchange chromatographic processes are discussed. (C) 1997 Elsevier Science B.V.