ENZYMATIC-SYNTHESIS OF SORBITAN ESTERS USING A LOW-BOILING-POINT AZEOTROPE AS A REACTION SOLVENT

Authors
SARNEY DB BARNARD MJ VIRTO M VULFSON EN
Citation
Db. Sarney et al., ENZYMATIC-SYNTHESIS OF SORBITAN ESTERS USING A LOW-BOILING-POINT AZEOTROPE AS A REACTION SOLVENT, Biotechnology and bioengineering, 54(4), 1997, pp. 351-356
Citations number
30
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology and bioengineering → ACNP
ISSN journal
00063592
Volume
54
Issue
4
Year of publication
1997
Pages
351 - 356
Database
ISI
SICI code
0006-3592(1997)54:4<351:EOSEUA>2.0.ZU;2-L
Abstract
Sorbitan esters were prepared by controlled dehydration of sorbitol fo llowed by lipase-catalyzed esterification of the resulting ''sorbitan. '' The reaction was carried out in azeotropic mixtures of tert-butanol /n-hexane. A partial phase diagram to determine the temperature requir ed for the distillation of the azeotrope at a given ratio of the solve nts was constructed. The effect of varying concentrations of the two s olvents on the rate of esterification and the monoester/diester ratio of the final product was investigated in detail. (C) 1997 John Wiley & Sons, Inc.