SYNTHESIS OF THIOREDOXIN PARTIAL SEQUENCES ON 1,6-HEXANEDIOL DIACRYLATE (HDODA)-CROSS-LINKED POLYSTYRENE RESIN

Citation
Jt. Varkey et Vnr. Pillai, SYNTHESIS OF THIOREDOXIN PARTIAL SEQUENCES ON 1,6-HEXANEDIOL DIACRYLATE (HDODA)-CROSS-LINKED POLYSTYRENE RESIN, The journal of peptide research, 51(1), 1998, pp. 49-54
Citations number
20
Categorie Soggetti
Biology
ISSN journal
1397002X
Volume
51
Issue
1
Year of publication
1998
Pages
49 - 54
Database
ISI
SICI code
1397-002X(1998)51:1<49:SOTPSO>2.0.ZU;2-K
Abstract
The continued and rapid discoveries of new peptides with interesting b iological functions have created an unprecedented demand for the chemi cal synthesis of peptides required for structure-function correlations . Several strategic improvements have been suggested and tested to mee t the demand for peptides in high purity and quantity. This article de scribes the synthesis of three partial sequences of thioredoxin, a nat urally occurring sulfur-reducing protein containing 108 amino acid res idues, on a newly developed flexible, cross-linked polystyrene support (2% polystyrene cross-linked with 1,6-hexanediol diacrylate) using th e standard solid-phase methodology. The protected peptides were cleave d from the polymeric support by trifluoroacetic acid and purified by c hromatography. The free peptides were shown to be homogeneous by high- performance liquid chromatography and were characterized by amino acid analysis and circular dichroism. The circular dichroism measurement r evealed that the peptides possess a helical conformation. From the yie ld and purity of the peptides obtained, it was inferred that the favor able swelling and solvation characteristics of the support facilitated effective synthesis. (C) Munksgaard 1997.