Jht. Luong et Al. Nguyen, ACHIRAL SELECTIVITY IN CYCLODEXTRIN-MODIFIED CAPILLARY ELECTROPHORESIS, Journal of chromatography, 792(1-2), 1997, pp. 431-444
Torus-shaped, circular, and hydrophilic cyclodextrins (CD) have been f
requently used in capillary electrophoresis (CE) as buffer modifiers t
o effect chiral separation of enantiomers of drugs and specialty chemi
cals. Although less common, both neutral and charged cyclodextrins hav
e also been exploited in CE to optimize the achiral separations of pep
tides, proteins, small molecules and a variety of positional isomers.
Nonionic CDs are only useful for separations of net charged analytes t
hrough judicious partitioning of such guest molecules into their hydro
phobic cavity of the former. However, they can be used with a surfacta
nt for an effective resolution of neutral solutes as a result of a dif
ferential partitioning of such solutes in the micellar and the cyclode
xtrin-modified buffer phase. Ionic cyclodextrins, particularly, negati
vely charged derivatives with their own electrophoretic mobilities, in
crease the separation window and enable better resolution of analytes
which weakly complex with or are poorly differentiated by neutral cycl
odextrins. (C) 1997 Elsevier Science B.V.