DETERMINATION OF THE 5 MAJOR OPIUM-ALKALOIDS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON A BASE-DEACTIVATED STATIONARY-PHASE

Authors
KRENN L GLANTSCHNIG S SORGNER U
Citation
L. Krenn et al., DETERMINATION OF THE 5 MAJOR OPIUM-ALKALOIDS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON A BASE-DEACTIVATED STATIONARY-PHASE, Chromatographia, 47(1-2), 1998, pp. 21-24
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
47
Issue
1-2
Year of publication
1998
Pages
21 - 24
Database
ISI
SICI code
0009-5893(1998)47:1-2<21:DOT5MO>2.0.ZU;2-U
Abstract
A reversed phase HPLC method for the separation of the five major alka loids from Papaver somniferum L., morphine, codeine, thebaine, papaver ine and noscapine, has been developed and validated. By use of a base- deactivated silica-based stationary phase excellent peak shape was ach ieved for each substance. The five alkaloids were quantified by intern al standardization within 20 min and with good precision. The method i s applicable to opium and to poppy straw.