Bioelectrical impedance analysis (BIA) was used to assess body composi
tion in rats fed on either standard laboratory diet or on a high-fat d
iet designed to induce obesity. Bioelectrical impedance analysis predi
ctions of total body water and thus fat-free mass (FFM) for the group
mean values were generally within 5% of the measured values by tritiat
ed water ((H2O)-H-3) dilution. The limits of agreement for the procedu
re were, however, large, approximately +/-25%, limiting the applicabil
ity of the technique for measurement of body composition in individual
animals.