PHYSICAL IDENTIFICATION OF A CHROMOSOMAL LOCUS ENCODING BIOSYNTHETIC GENES FOR THE LIPOPEPTIDE CALCIUM-DEPENDENT ANTIBIOTIC (CDA) OF STREPTOMYCES-COELICOLOR A3(2)

Authors
CHONG PP PODMORE SM KIESER HM REDENBACH M TURGAY K MARAHIEL M HOPWOOD DA SMITH CP
Citation
Pp. Chong et al., PHYSICAL IDENTIFICATION OF A CHROMOSOMAL LOCUS ENCODING BIOSYNTHETIC GENES FOR THE LIPOPEPTIDE CALCIUM-DEPENDENT ANTIBIOTIC (CDA) OF STREPTOMYCES-COELICOLOR A3(2), Microbiology, 144, 1998, pp. 193-199
Citations number
33
Categorie Soggetti
Microbiology
Journal title
Microbiology → ACNP
ISSN journal
13500872
Volume
144
Year of publication
1998
Part
1
Pages
193 - 199
Database
ISI
SICI code
1350-0872(1998)144:<193:PIOACL>2.0.ZU;2-V
Abstract
Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approac h and mapped to a single similar to 35 kb segment. In integrative tran sformation experiments, DNA fragments from this region disrupted produ ction of the calcium-dependent antibiotic (CDA), and had sequences cha racteristic of non-ribosomal peptide synthetases, thus proving that th e cda locus had been cloned.