DETERMINATION OF CIS AND TRANS ISOMERS OF TRAMADOL HYDROCHLORIDE BY CAPILLARY-ZONE-ELECTROPHORESIS

Cis and trans isomers of tramadol hydrochloride were separated by capi llary zone electrophoresis (CZE) in a fused-silica capillary with 40 m mol/L of berate as a carrier, The sample concentration-peak area plots were straight lines in the range 10-250 mu g/mL for both isomers, Bas ed on the electropherogram, the relationship between the structure of the two isomers and the elution order has been discussed, It is consid ered that the trans isomer has larger hydrodynamic radius than the cis Isomer and lower electric charge density, The elution order can be pr edicted only according to the direction of electrophoresis and electro endo-osmotic flow, The technique was successfully applied to the indus trial production and the research for utilization of industrial waste material. (C) 1998 John Wiley & Sons, Ltd.