DNA-ADDUCTS IN MOUSE TESTIS AND LUNG AFTER INHALATION EXPOSURE TO 1,3-BUTADIENE

1,3-Butadiene is a widely used industrial chemical and also an environ mental contaminant. Recent findings have shown that butadiene can also be a male germ cell mutagen. In this study, DNA adduct formation in t estis and lung has been explored by using N7-alkylated guanines as a m arker of biological effective dose, The adducts measured were the four structurally different guanine N7-adducts alkylated by butadienemonoe poxide, the main metabolite of 1,3-butadiene. This study demonstrates the dose-dependent adduct formation in lung and testis. At lower expos ures (50 and 200 ppm) the adduct levels were about the same in the two organs, but at 500 ppm the adduct level was significantly (p < 0.03) higher in testis than in lung. The enantiomeric composition of the add ucts detected was also different. In lung, all 4 possible adducts were present (S-C-1 '' dominating, 49%), but in testis only two out of fou r adducts were detected (S-C-2 '' being the most abundant adduct, 71%) . These novel observations indicate that the DNA repair is different i n these two organs studied and that heritable genetic effects observed may be mediated through the DNA adducts. (C) 1998 Elsevier Science B. V.