DEVELOPMENT OF AN AMPEROMETRIC BIOSENSOR BASED ON ACETYLCHOLINE ESTERASE COVALENTLY BOUND TO A NEW SUPPORT MATERIAL

Authors
KHAYYAMI M PITA MTP GARCIA NP JOHANSSON G DANIELSSON B LARSSON PO
Citation
M. Khayyami et al., DEVELOPMENT OF AN AMPEROMETRIC BIOSENSOR BASED ON ACETYLCHOLINE ESTERASE COVALENTLY BOUND TO A NEW SUPPORT MATERIAL, Talanta, 45(3), 1998, pp. 557-563
Citations number
16
Categorie Soggetti
Chemistry Analytical
Journal title
Talanta
ISSN journal
00399140 → ACNP
Volume
45
Issue
3
Year of publication
1998
Pages
557 - 563
Database
ISI
SICI code
0039-9140(1998)45:3<557:DOAABB>2.0.ZU;2-E
Abstract
A new type of amperometric biosensor based on immobilised acetylcholin e esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using ace tylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 mon th. (C) 1998 Elsevier Science B.V.