EFFECT OF VIABILITY OF ACTINOMYCETE SPORES ON THEIR ABILITY TO STIMULATE PRODUCTION OF NITRIC-OXIDE AND REACTIVE OXYGEN SPECIES IN RAW264.7MACROPHAGES

Citation
Mr. Hirvonen et al., EFFECT OF VIABILITY OF ACTINOMYCETE SPORES ON THEIR ABILITY TO STIMULATE PRODUCTION OF NITRIC-OXIDE AND REACTIVE OXYGEN SPECIES IN RAW264.7MACROPHAGES, Toxicology, 124(2), 1997, pp. 105-114
Citations number
28
Journal title
ISSN journal
0300483X
Volume
124
Issue
2
Year of publication
1997
Pages
105 - 114
Database
ISI
SICI code
0300-483X(1997)124:2<105:EOVOAS>2.0.ZU;2-U
Abstract
Spores of actinomycetes, mesophilic gram-positive bacteria, isolated f rom moldy houses, induced the expression of inducible NO-synthase (iNO S) with a subsequent NO-production in RAW264.7 macrophages. No differe nces were detected between production of nitric oxide (NO) by alive or irradiated spores of different strains of Actinomycetes sp. or Strept omyces sp. Moreover, a significant production of reactive oxygen speci es (ROS) occurred in the macrophages after their stimulation both by a live and irradiation killed spores of actinomycetes. However, ROS-resp onses in macrophage induced by dead spores were significantly lower co mpared to those induced by alive spores. The cytotoxicity of the spore s of different actinomycetes differed widely. The production of NO and ROS did not depend directly on the viability of the spores, suggestin g an important role for cell wall components in the activation of the cells. (C) 1997 Elsevier Science Ireland Ltd.