P. Sancheztorres et al., HETEROLOGOUS EXPRESSION OF A CANDIDA-MOLISCHIANA ANTHOCYANIN-BETA-GLUCOSIDASE IN A WINE YEAST-STRAIN, Journal of agricultural and food chemistry, 46(1), 1998, pp. 354-360
A recombinant wine yeast strain expressing the Candida molischiana bgl
n gene encoding a beta-glucosidase/anthocyanase under the control of t
he Saccharomyces cerevisiae actin gene promoter has been constructed.
The corresponding protein, BGLN, was mainly located on the cell wall.
BGLN was purified in a single chromotagraphic step, and different phys
icochemical and kinetic properties have been determined. BGLN showed m
aximum activity against the artificial substrate p-nitrophenyl beta-D-
glucopyranoside. It also hydrolyzed salicin, p-nitrophenyl beta-D-xylo
side, cellobiose, and arbutin to a lesser extent. Fructose and SO2 did
not affect enzyme activity, which was activated by ethanol, while glu
cose was a strong competitive inhibitor. The purified BGLN showed a no
vel anthocyanase decolorizing capability on red wines. This anthocyana
se activity was readily observed during microvinification experiments.
However, the physicochemical characteristics of the wines obtained wi
th the recombinant wine yeast strain were indistinguishable from those
obtained with the parental strain.