ENZYMATIC HISTOCHEMICAL-LOCALIZATION OF ALDEHYDE OXIDASE IN RAT-LIVERBY THE TETRAZOLIUM METHOD

The enzymatic histochemical localization of aldehyde oxidase in the ra t liver lobule was investigated by use of a tissue protectant, polyvin yl alcohol, with tetra-nitro BT as the final electron acceptor, This m ethod demonstrated the presence of aldehyde oxidase activity in the cy toplasm of liver cells. Distribution of aldehyde oxidase activity in t he liver was uneven, being seen mainly in the pericentral rather than the periportal area as with xanthine oxidase. The reaction was not inh ibited by TEI-6720 but was markedly inhibited by benzamidine. Results were consistent with those of immunohistochemical localization studies of this enzyme.