R. Fabbri et al., FOLLICULAR-FLUID AND HUMAN GRANULOSA-CELL CULTURES - INFLUENCE ON SPERM KINETIC-PARAMETERS, HYPERACTIVATION, AND ACROSOME REACTION, Fertility and sterility, 69(1), 1998, pp. 112-117
Objective: To evaluate the influence of human granulosa cell (GC) cult
ures and follicular fluid (FF) on sperm kinetic parameters, hyperactiv
ation, and the acrosome reaction compared with the influence of human
tubal fluid (HTF) and Ham's F-10 medium. Design: Sperm kinetic paramet
ers, hyperactivation, and the acrosome reaction were evaluated after 6
hours of incubation in HTF, Ham's F-10 medium, FF, and GC cultures. S
etting: Infertility and In Vitro Fertilization Centre, Reproductive En
docrinology Unit, Institute of Obstetrics and Gynaecology. Patient(s):
Sixteen normal semen samples. Intervention(s): Sperm kinetic paramete
rs and hyperactivation were analyzed using an automated videomicrograp
hy system, the acrosome reaction was performed using a triple-stain te
chnique, and progesterone and 17OH-progesterone levels were measured w
ith the use of commercially available kits. Main Outcome Measure(s): S
perm kinetic parameters, hyperactivation, acrosome reaction. Result(s)
: The percentage of-motile sperm, the mean curvilinear velocity, and t
he mean of the maximum amplitude of lateral head movement were increas
ed significantly after 6 hours of incubation in FF or GC cultures comp
ared with incubation in HTF or Ham's F-10 medium, whereas the mean lin
earity was decreased significantly. Follicular fluid and GC cultures s
ignificantly increased hyperactivation and the acrosome reaction compa
red with the values obtained using HTF and Ham's F-10 medium. Progeste
rone and 17OH-progesterone levels were increased significantly after i
ncubation in FF and GC cultures compared with HTF and Ham's F-10 mediu
m. Conclusion(s): Follicular fluid and GC cultures increase sperm moti
lity parameters, hyperactivation, and the acrosome reaction. This effe
ct may be related to GC detoxification of the microenvironement of the
microenvironement or GC secretion of peptides, glycoproteins, growth
factors (insulin-like growth factors 1 and 2), or steroids (progestero
ne and 17OH-progesterone). (C) 1998 by American Society for Reproducti
ve Medicine.