Naturally-occurring variations in the abundance of stable isotopes of
carbon, nitrogen, oxygen, and other elements in plants have been repor
ted and are now used to understand various physiological processes in
plants. Boron (B) isotopic variation in several plant species has been
documented, but no determination as to whether plants fractionate the
stable isotopes of boron, B-11 and B-10, has been made. Here, we repo
rt that plants with differing B requirements (wheat, corn and broccoli
) fractionated boron. The whole plant was enriched in B-11 relative to
the nutrient solution, and the leaves were enriched in B-11 and the s
tem in B-11 relative to the xylem sap. Although at present, a mechanis
tic role for boron in plants is uncertain, potential fractionating mec
hanisms are discussed.