EFFECT OF METHANOBREVIBACTER SP MF1 INOCULATION ON GLYCOSIDE HYDROLASE AND POLYSACCHARIDE DEPOLYMERASE ACTIVITIES, WHEAT-STRAW DEGRADATION AND VOLATILE FATTY-ACID CONCENTRATIONS IN THE RUMEN OF GNOTOBIOTICALLY-REARED LAMBS
G. Fonty et al., EFFECT OF METHANOBREVIBACTER SP MF1 INOCULATION ON GLYCOSIDE HYDROLASE AND POLYSACCHARIDE DEPOLYMERASE ACTIVITIES, WHEAT-STRAW DEGRADATION AND VOLATILE FATTY-ACID CONCENTRATIONS IN THE RUMEN OF GNOTOBIOTICALLY-REARED LAMBS, Anaerobe, 3(6), 1997, pp. 383-389
Four naturally born lambs were placed in sterile isolators 24 h after
birth before the natural establishment of cellulolytic microorganisms
and archaea methanogens. At the age of 6 weeks they were inoculated wi
th pure cultures of the strains FD1 and 007 of Ruminococcus flavefacie
ns and at the age of 4 months with a pure culture of Methanobrevibacte
r sp. MF1. Following the establishment of MF1, the population of X. fl
avefaciens slightly increased in the rumen of the four lambs, there wa
s also an increase in straw degradation, in the activity of some glyco
side and polysaccharide hydrolases of the adherent microbial populatio
ns and in the concentration of acetate in ruminal contents. (C) 1997 A
cademic Press.