H. Donmez et al., DETECTION AND TYPING OF HUMAN-PAPILLOMAVIRUS DNAS BY RESTRICTION-ENDONUCLEASE MAPPING OF THE PCR PRODUCTS, Israel journal of medical sciences, 33(12), 1997, pp. 789-793
Human papillomavirus (HPV) has been implicated strongly with human cer
vical, anal and penile cancers. The polymerase chain reaction (PCR) wa
s used to detect HPV in cervical specimens of 88 women working at the
public whorehouse. Using consensus primers which encode the L1 region
of the HPV genome, the presence of HPV DNA was demonstrated in 2 speci
mens. Restriction endonuclease digestion of the amplified products was
carried out for accurate typing. Samples which were positive by L1 PC
R were digested with Hae III, BstN I and Dde I restriction enzymes. Th
e patterns produced by digestion were identified as HPV types 6b and 1
6.