ACTIVATION OF SYK IN AN IMMATURE B-CELL LINE DOES NOT REQUIRE LYN ACTIVITY

BKS-2 is an immature B cell lymphoma that undergoes apoptotic cell dea th when signaled via its surface IgM receptor. To study the signaling components of surface IgM mediated apoptosis in B lymphoma cells, we g enerated mutants of BKS-2 that were resistant to anti-IgM induced apop tosis. One mutant cell line, 1.B5, did not undergo apoptotic cell deat h upon treatment with anti-IgM antibodies and also did not exhibit ele vation of intracellular Ca2+ in response to cross-linking of surface I gM. This appeared to be due to a defect in protein tyrosine kinase (PT K) activity since fewer proteins were tyrosine phosphorylated in the m utant cells stimulated with anti-IgM when compared to wild type BKS-2. Subsequently, we showed that protein tyrosine kinases lyn and blk wer e inducibly tyrosine phosphorylated in the wild type BKS-2 but not in 1.B5 mutant cells in response to anti-IgM. Also the kinase activity of lyn was elevated in the wild type but not in mutant cells upon trigge ring through surface IgM. Furthermore, tyrosine phosphorylation of CD 19, a known substrate of lyn, was inducible in anti-IgM stimulated BKS -2 cells but severely reduced in 1.B5 cells. In contrast, kinase activ ity of another src kinase, blk, was increased on anti-IgM stimulation in both wild type and mutant cells. Surprisingly, syk, a non-src prote in tyrosine kinase important for surface IgM mediated signaling, was t yrosine phosphorylated in the lyn deficient mutant cells as well as in the wild type BKS-2 cells. Furthermore, anti-IgM induced increase in kinase activity of syk was similar in the mutant and wild type cells. Thus, in contrast to other studies that propose syk to be a downstream target of src family kinases, syk may act upstream of lyn in immature B cells. Consistent with a functional syk, its target, phospholipase gamma 2 (PLC-gamma 2) was normally tyrosine phosphorylated in mutant c ells. (C) 1998 Elsevier Science Ltd. All rights reserved.