EFFECT OF ASP-97-]GLU SUBSTITUTION ON PH-DEPENDENCE OF CATALYSIS BY INORGANIC PYROPHOSPHATASE OF ESCHERICHIA-COLI

Authors
FABRICHNIY IP LAHTI R BAYKOV AA
Citation
Ip. Fabrichniy et al., EFFECT OF ASP-97-]GLU SUBSTITUTION ON PH-DEPENDENCE OF CATALYSIS BY INORGANIC PYROPHOSPHATASE OF ESCHERICHIA-COLI, Biochemistry, 62(9), 1997, pp. 946-950
Citations number
19
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
62
Issue
9
Year of publication
1997
Pages
946 - 950
Database
ISI
SICI code
0006-2979(1997)62:9<946:EOASOP>2.0.ZU;2-G
Abstract
Substitution of Glu for the evolutionarily ! conserved Asp-97 in the a ctive site of Escherichia coil inorganic pyrophosphatase increases the apparent pK(a) of the essential acidic group controlling the catalyti c constant for pyrophosphate hydrolysis!. In combination with previous ly reported data, this fact suggests that activity!. decreases in alka line medium because of decreased rats of the rt release of the first m olecule of the product phosphate from the active site.