ISOFORMS OF SERRATIA-MARCESCENS NUCLEASE - THE ROLE OF MG2+ IN THE HYDROLYSIS MECHANISM

Authors
FILIMONOVA MN GUBSKAYA VP NURETDINOV IA BENEDIK MJ BOGOMOLNAYA LM ANDREEVA MA LESHCHINSKAYA IB
Citation
Mn. Filimonova et al., ISOFORMS OF SERRATIA-MARCESCENS NUCLEASE - THE ROLE OF MG2+ IN THE HYDROLYSIS MECHANISM, Biochemistry, 62(9), 1997, pp. 983-988
Citations number
30
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
62
Issue
9
Year of publication
1997
Pages
983 - 988
Database
ISI
SICI code
0006-2979(1997)62:9<983:IOSN-T>2.0.ZU;2-D
Abstract
Structural and functional differences between isoforms Sm1 and Sm2, a lack of influence of free Mg2+ on the isoform structures, formation of DNA-magnesium complex serving with great probability as a real substr ate for the nuclease has been summarized on the basis of experimental data. Mg2+ forming a complex with phosphate groups of DNA are supposed to further increase the electrophilicity of the phosphorus atoms besi des causing a conformational change of the substrate.