ACTIVATION OF LIPID-METABOLISM OF NUCLEI AND CHROMATIN IN THYMOCYTES OF RATS SUBJECTED TO CHRONIC LOW-DOSE INTENSITY GAMMA-IRRADIATION

Contents and radioactivity (as incorporation of [2-C-14]acetate) of th ymocyte nuclear and chromatin lipids were investigated in rats subject ed to chronic gamma-irradiation in a dose 10 Gy at the intensity of 12 .9 cGy/day. The incorporation of label into total fraction of lipids o f nuclei and chromatin and into total phospholipid fraction of thymocy te nuclei increased. However, in the whole cells differences in radioa ctivity of these fractions were not found. The amount of fatty acids i n thymocytes from irradiated rats was decreased but synthesis of fatty acids was activated. The amount of fatty acids in nuclei and chromati n remained unchanged compared with control, whereas radioactivity was significantly higher than in the whole cells. In irradiated thymocytes the amounts of phosphatidylserine in nuclei and phosphatidylserine an d phosphatidylinositol in chromatin were increased. The incorporation of [2-C-14]acetate into proteins increased in thymocytes of irradiated rats. The role of lipid metabolism in genome functioning is discussed .