FACTORS THAT AFFECT THE EXPRESSION OF BETA-AMYLOID PRECURSOR PROTEIN IMMUNOREACTIVITY IN THE RAT RETINA

Expression of beta-amyloid precursor protein immunoreactivity in the r at retina was studied after intravitreal injection of substances known to influence neural function in different ways. The substances were t he excitatory amino acid glutamate, the inflammatory agent lipopolysac charide, the depolarizing agent potassium chloride, and the potassium channel blocker barium chloride. In comparison with controls, more bet a-amyloid precursor protein immunoreactivity was observed in the radia l process of Muller glial cells 24 hours after injection of glutamate or lipopolysaccharide. In contrast, administration of barium chloride greatly reduced immunostaining in Muller cells. Further an increase in immunostaining was observed in the inner and outer plexiform layers i n retinas treated with any of the 3 chemicals, and in blood vessels af ter injection of glutamate and lipopolysaccharide. These observations suggest that multiple but specific signaling pathways are involved in regulating expression of beta-amyloid precursor protein in distinct ce ll types and regions in the retina.