THE ROLE OF GANGLIOSIDE GM(3) IN THE MODULATION OF CONFORMATION AND ACTIVITY OF SARCOPLASMIC-RETICULUM CA2-ATPASE()

Authors
YANG FY WANG LH YANG XY TSUI ZC TU YP
Citation
Fy. Yang et al., THE ROLE OF GANGLIOSIDE GM(3) IN THE MODULATION OF CONFORMATION AND ACTIVITY OF SARCOPLASMIC-RETICULUM CA2-ATPASE(), Biophysical chemistry, 68(1-3), 1997, pp. 137-146
Citations number
36
Categorie Soggetti
Biophysics,Biology,"Chemistry Physical
Journal title
Biophysical chemistry → ACNP
ISSN journal
03014622
Volume
68
Issue
1-3
Year of publication
1997
Pages
137 - 146
Database
ISI
SICI code
0301-4622(1997)68:1-3<137:TROGGI>2.0.ZU;2-6
Abstract
Rabbit sarcoplasmic reticulum does contain trace amounts of gangliosid es, and the main species is GM(3). Incorporation of GM(3) into the SR vesicles or addition of it to the soybean phospholipid used for recons titution of proteoliposomes obviously increased ATP hydrolysis, as wel l as, Ca2+ uptake activity of sarcoplasmic reticulum Ca2+-ATPase. Conf ormation changes of Ca2+-ATPase induced by GM(3) were also observed by circular dichroism, intrinsic fluorescence and fluorescence quenching measurements. (C) 1997 Elsevier Science B.V.