PRELIMINARY-OBSERVATIONS ON RESPONSES OF EMBRYONIC AND LARVAL PACIFICHERRING, CLUPEA-PALLASI, TO NEUTRAL FRACTION BIODEGRADATION PRODUCTS OF WEATHERED ALASKA NORTH SLOPE OIL

Citation
Dp. Middaugh et al., PRELIMINARY-OBSERVATIONS ON RESPONSES OF EMBRYONIC AND LARVAL PACIFICHERRING, CLUPEA-PALLASI, TO NEUTRAL FRACTION BIODEGRADATION PRODUCTS OF WEATHERED ALASKA NORTH SLOPE OIL, Archives of environmental contamination and toxicology, 34(2), 1998, pp. 188-196
Citations number
27
Categorie Soggetti
Toxicology,"Environmental Sciences
ISSN journal
00904341
Volume
34
Issue
2
Year of publication
1998
Pages
188 - 196
Database
ISI
SICI code
0090-4341(1998)34:2<188:POROEA>2.0.ZU;2-C
Abstract
Weathered Alaska North Slope crude oil (ANS 521) was subjected to biod egradation in vigorously stirred incubations for 14 days at 15 +/- 1 d egrees C in 20 parts per thousand salinity sterilized seawater, amende d with nutrients and inoculated with a hydrocarbon-degrading microorga nism (EI2V) isolated from an oil-contaminated beach in Prince William Sound, Alaska. A total of 13.7 mg/L water-soluble neutral fraction (WS F) was recovered from the incubation of weathered ANS 521. Toxicity/te ratogenicity tests were conducted with WSF recovered from the biodegra dation system using embryonic and larval Pacific herring, Clupea palla si. Exposures fl;ere begun at 4, 48, and 96 h postfertilization of her ring eggs. Exposure concentrations were 1, 10, and 100% of the origina l concentration of WSF recovered from incubations (redissolved in 20 p arts per thousand salinity sterile seawater at 15 +/- 1 degrees C)., S terile 20 parts per thousand salinity seawater without the addition of redissolved neutral fraction was used as a control, Significant (p le ss than or equal to 0.05) embryo mortality or teratogenic responses we re observed at WSF concentrations of 10 and 100%. On days 5 through 8 of embryogenesis, counts of heart contraction rates were significantly lower (p less than or equal to: 0.05) at the 100% WSF concentration f or embryos exposed beginning at 4 and 48 h postfertilization. Grow-out of larvae from selected exposures was conducted, High mortality was n oted in larvae exposed to the 10% WSF concentration beginning at 4 and ?8 h postfertilization., Most of these larvae died 5 to 8 days after hatching when they elicited vertebral displacements at a time concurre nt with the onset of feeding behavior.