PRODUCTION AND PROPERTIES OF A CELLOBIOSE-OXIDIZING ENZYME FROM A NEWLY ISOLATED CELLULOLYTIC BACTERIUM CYTOPHAGA SP. LX-7

A cell-bound cellobiose-oxidizing enzyme was produced by cellulolytic Cytophaga LX-7. It was found that both sp. the cellulosic substrates a nd the soluble carbohydrate substrates tested promoted the production of the cellobiose-oxidizing enzyme, and the highest specific activitie s were obtained with cellulose powder MN300, carboxymethylcellulose CM 22, maltose and cellobiose. Among the nitrogen sources examined, pepto ne gave the best cellobiose-oxidizing enzyme production, whereas inorg anic nitrogen sources gave very poor growth. The medium buffered with Tris/HCl, pH 7.1, yielded the highest levels of cellobiose-oxidizing e nzyme activity and the temperature optimum for crude enzyme activity w as 40 degrees C.